Rhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis.

TitleRhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis.
Publication TypeJournal Article
Year of Publication2016
AuthorsHeinonen S, Jartti T, Garcia C, Oliva S, Smitherman C, Anguiano E, Piters WAA de Ste, Vuorinen T, Ruuskanen O, Dimo B, Suarez NM, Pascual V, Ramilo O, Mejias A
JournalAm J Respir Crit Care Med
Volume193
Issue7
Pagination772-82
Date Published2016 Apr 01
ISSN1535-4970
KeywordsAsymptomatic Infections, Biomarkers, Blood Cell Count, Female, Finland, Gene Expression Profiling, Humans, Infant, Male, Ohio, Picornaviridae Infections, Prospective Studies, Real-Time Polymerase Chain Reaction, Respiratory Tract Infections, Rhinovirus, Spain, Texas
Abstract

RATIONALE: Rhinoviruses (RVs) are a major cause of symptomatic respiratory tract infection in all age groups. However, RVs can frequently be detected in asymptomatic individuals.

OBJECTIVES: To evaluate the ability of host transcriptional profiling to differentiate between symptomatic RV infection and incidental detection in children.

METHODS: Previously healthy children younger than 2 years old (n = 151) were enrolled at four study sites and classified into four clinical groups: RV- healthy control subjects (n = 37), RV+ asymptomatic subjects (n = 14), RV+ outpatients (n = 30), and RV+ inpatients (n = 70). Host responses were analyzed using whole-blood RNA transcriptional profiles.

MEASUREMENTS AND MAIN RESULTS: RV infection induced a robust transcriptional signature, which was validated in three independent cohorts and by quantitative real-time polymerase chain reaction with high prediction accuracy. The immune profile of symptomatic RV infection was characterized by overexpression of innate immunity and underexpression of adaptive immunity genes, whereas negligible changes were observed in asymptomatic RV+ subjects. Unsupervised hierarchical clustering identified two main clusters of subjects. The first included 93% of healthy control subjects and 100% of asymptomatic RV+ subjects, and the second comprised 98% of RV+ inpatients and 88% of RV+ outpatients. Genomic scores of healthy control subjects and asymptomatic RV+ children were similar and significantly lower than those of RV+ inpatients and outpatients (P < 0.0001).

CONCLUSIONS: Symptomatic RV infection induced a robust and reproducible transcriptional signature, whereas identification of RV in asymptomatic children was not associated with significant systemic transcriptional immune responses. Transcriptional profiling represents a useful tool to discriminate between active infection and incidental virus detection.

DOI10.1164/rccm.201504-0749OC
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https://www.ncbi.nlm.nih.gov/pubmed/26571305?dopt=Abstract

Alternate JournalAm. J. Respir. Crit. Care Med.
PubMed ID26571305
PubMed Central IDPMC4824929
Grant ListU19 AI089987 / AI / NIAID NIH HHS / United States
U19AI089987 / AI / NIAID NIH HHS / United States

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