| Title | Protocol for cross-platform characterization of human and murine extracellular vesicles and particles. |
| Publication Type | Journal Article |
| Year of Publication | 2024 |
| Authors | Bojmar L, Kim HSang, Sugiura K, Heissel S, Lucotti S, Cioffi M, Johnson KEnnu, Cohen-Gould L, Zhang H, Molina H, Matei IR, Lyden D, Hoshino A |
| Journal | STAR Protoc |
| Volume | 5 |
| Issue | 1 |
| Pagination | 102754 |
| Date Published | 2024 Mar 15 |
| ISSN | 2666-1667 |
| Keywords | Animals, Blotting, Western, Computational Biology, Extracellular Vesicles, Humans, Mass Spectrometry, Mice, Proteomics |
| Abstract | Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. We describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross-validation across different platforms. For complete details on the use and execution of this protocol, please refer to Hoshino et al.1. |
| DOI | 10.1016/j.xpro.2023.102754 |
| Custom 1 | |
| Alternate Journal | STAR Protoc |
| PubMed ID | 38096060 |
| PubMed Central ID | PMC10762520 |
| Grant List | U54 CA163120 / CA / NCI NIH HHS / United States U54 CA163117 / CA / NCI NIH HHS / United States R01 CA218513 / CA / NCI NIH HHS / United States U19 AI144301 / AI / NIAID NIH HHS / United States UL1 TR002384 / TR / NCATS NIH HHS / United States U01 CA210240 / CA / NCI NIH HHS / United States UL1 TR000457 / TR / NCATS NIH HHS / United States R35 CA232093 / CA / NCI NIH HHS / United States R01 CA207983 / CA / NCI NIH HHS / United States S10 RR027699 / RR / NCRR NIH HHS / United States R01 CA169416 / CA / NCI NIH HHS / United States U01 CA169538 / CA / NCI NIH HHS / United States U01 CA224175 / CA / NCI NIH HHS / United States |