| Title | Non-criteria antiphospholipid antibodies and calprotectin as potential biomarkers in pediatric antiphospholipid syndrome. |
| Publication Type | Journal Article |
| Year of Publication | 2024 |
| Authors | Sloan EE, Kmetova K, NaveenKumar SK, Kluge L, Chong E, Hoy CK, Yalavarthi S, Sarosh C, Baisch J, Walters L, Nassi L, Fuller J, Turnier JL, Pascual V, Wright TB, Madison JA, Knight JS, Zia A, Zuo Y |
| Journal | Clin Immunol |
| Volume | 261 |
| Pagination | 109926 |
| Date Published | 2024 Apr |
| ISSN | 1521-7035 |
| Keywords | Antibodies, Antiphospholipid, Antiphospholipid Syndrome, beta 2-Glycoprotein I, Biomarkers, Child, Humans, Immunoglobulin G, Immunoglobulin M, Leukocyte L1 Antigen Complex, Prothrombin |
| Abstract | Our study aimed to evaluate the presence, clinical associations, and potential mechanistic roles of non-criteria antiphospholipid antibodies (aPL) and circulating calprotectin, a highly stable marker of neutrophil extracellular trap release (NETosis), in pediatric APS patients. We found that 79% of pediatric APS patients had at least one non-criteria aPL at moderate-to-high titer. Univariate logistic regression demonstrated that positive anti-beta-2 glycoprotein I domain 1 (anti-D1) IgG (p = 0.008), anti-phosphatidylserine/prothrombin (aPS/PT) IgG (p < 0.001), and aPS/PT IgM (p < 0.001) were significantly associated with venous thrombosis. Positive anti-D1 IgG (p < 0.001), aPS/PT IgG (p < 0.001), and aPS/PT IgM (p = 0.001) were also associated with non-thrombotic manifestations of APS, such as thrombocytopenia. Increased levels of calprotectin were detected in children with APS. Calprotectin correlated positively with absolute neutrophil count (r = 0.63, p = 0.008) and negatively with platelet count (r = -0.59, p = 0.015). Mechanistically, plasma from pediatric APS patients with high calprotectin levels impaired platelet viability in a dose-dependent manner. |
| DOI | 10.1016/j.clim.2024.109926 |
| Custom 1 | |
| Alternate Journal | Clin Immunol |
| PubMed ID | 38355030 |