Medulloblastoma subgroups remain stable across primary and metastatic compartments.

TitleMedulloblastoma subgroups remain stable across primary and metastatic compartments.
Publication TypeJournal Article
Year of Publication2015
AuthorsWang X, Dubuc AM, Ramaswamy V, Mack S, Gendoo DMA, Remke M, Wu X, Garzia L, Luu B, Cavalli F, Peacock J, López B, Skowron P, Zagzag D, Lyden DC, Hoffman C, Cho Y-J, Eberhart C, MacDonald T, Li X-N, Van Meter T, Northcott PA, Haibe-Kains B, Hawkins CE, Rutka JT, Bouffet E, Pfister SM, Korshunov A, Taylor MD
JournalActa Neuropathol
Date Published2015 Mar
KeywordsAdolescent, Cerebellar Neoplasms, Child, Child, Preschool, Cluster Analysis, Female, Humans, Male, Medulloblastoma, Neoplasm Metastasis, Oligonucleotide Array Sequence Analysis, Transcriptome

Medulloblastoma comprises four distinct molecular variants with distinct genetics, transcriptomes, and outcomes. Subgroup affiliation has been previously shown to remain stable at the time of recurrence, which likely reflects their distinct cells of origin. However, a therapeutically relevant question that remains unanswered is subgroup stability in the metastatic compartment. We assembled a cohort of 12-paired primary-metastatic tumors collected in the MAGIC consortium, and established their molecular subgroup affiliation by performing integrative gene expression and DNA methylation analysis. Frozen tissues were collected and profiled using Affymetrix gene expression arrays and Illumina methylation arrays. Class prediction and hierarchical clustering were performed using existing published datasets. Our molecular analysis, using consensus integrative genomic data, establishes the unequivocal maintenance of molecular subgroup affiliation in metastatic medulloblastoma. We further validated these findings by interrogating a non-overlapping cohort of 19 pairs of primary-metastatic tumors from the Burdenko Neurosurgical Institute using an orthogonal technique of immunohistochemical staining. This investigation represents the largest reported primary-metastatic paired cohort profiled to date and provides a unique opportunity to evaluate subgroup-specific molecular aberrations within the metastatic compartment. Our findings further support the hypothesis that medulloblastoma subgroups arise from distinct cells of origin, which are carried forward from ontogeny to oncology.

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Alternate JournalActa Neuropathol.
PubMed ID25689980
PubMed Central IDPMC4333718
Grant ListR01CA159859 / CA / NCI NIH HHS / United States
R01 CA159859 / CA / NCI NIH HHS / United States
/ / Canadian Institutes of Health Research / Canada
R01 CA148699 / CA / NCI NIH HHS / United States
R01CA148699 / CA / NCI NIH HHS / United States

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