Longitudinal profiling of human blood transcriptome in healthy and lupus pregnancy.

TitleLongitudinal profiling of human blood transcriptome in healthy and lupus pregnancy.
Publication TypeJournal Article
Year of Publication2019
AuthorsHong S, Banchereau R, Maslow B-SL, Guerra MM, Cardenas J, Baisch J, D Branch W, T Porter F, Sawitzke A, Laskin CA, Buyon JP, Merrill J, Sammaritano LR, Petri M, Gatewood E, Cepika A-M, Ohouo M, Obermoser G, Anguiano E, Kim TWhan, Nulsen J, Nehar-Belaid D, Blankenship D, Turner J, Banchereau J, Salmon JE, Pascual V
JournalJ Exp Med
Date Published2019 05 06
KeywordsAdult, Biomarkers, Embryo Implantation, Female, Humans, Longitudinal Studies, Lupus Erythematosus, Systemic, Pre-Eclampsia, Pregnancy, Pregnancy Complications, Prospective Studies, RNA-Seq, Transcriptome

Systemic lupus erythematosus carries an increased risk of pregnancy complications, including preeclampsia and fetal adverse outcomes. To identify the underlying molecular mechanisms, we longitudinally profiled the blood transcriptome of 92 lupus patients and 43 healthy women during pregnancy and postpartum and performed multicolor flow cytometry in a subset of them. We also profiled 25 healthy women undergoing assisted reproductive technology to monitor transcriptional changes around embryo implantation. Sustained down-regulation of multiple immune signatures, including interferon and plasma cells, was observed during healthy pregnancy. These changes appeared early after embryo implantation and were mirrored in uncomplicated lupus pregnancies. Patients with preeclampsia displayed early up-regulation of neutrophil signatures that correlated with expansion of immature neutrophils. Lupus pregnancies with fetal complications carried the highest interferon and plasma cell signatures as well as activated CD4 T cell counts. Thus, blood immunomonitoring reveals that both healthy and uncomplicated lupus pregnancies exhibit early and sustained transcriptional modulation of lupus-related signatures, and a lack thereof associates with adverse outcomes.

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Alternate JournalJ. Exp. Med.
PubMed ID30962246
PubMed Central IDPMC6504211
Grant ListR01 AR049772 / AR / NIAMS NIH HHS / United States
P50 AR070594 / AR / NIAMS NIH HHS / United States
R01 AR043727 / AR / NIAMS NIH HHS / United States
R01 AR069572 / AR / NIAMS NIH HHS / United States
U19 AI082715 / AI / NIAID NIH HHS / United States

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