Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

TitleHeterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.
Publication TypeJournal Article
Year of Publication2017
AuthorsShi Z, Fujii K, Kovary KM, Genuth NR, Röst HL, Teruel MN, Barna M
JournalMol Cell
Date Published2017 Jul 06
KeywordsAnimals, Cell Line, Drosophila melanogaster, Drosophila Proteins, Embryonic Stem Cells, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Internal Ribosome Entry Sites, Protein Biosynthesis, Protein Interaction Maps, Ribosomal Proteins, Ribosomes, RNA Interference, RNA, Messenger, Transcriptome, Transfection

Emerging studies have linked the ribosome to more selective control of gene regulation. However, an outstanding question is whether ribosome heterogeneity at the level of core ribosomal proteins (RPs) exists and enables ribosomes to preferentially translate specific mRNAs genome-wide. Here, we measured the absolute abundance of RPs in translating ribosomes and profiled transcripts that are enriched or depleted from select subsets of ribosomes within embryonic stem cells. We find that heterogeneity in RP composition endows ribosomes with differential selectivity for translating subpools of transcripts, including those controlling metabolism, cell cycle, and development. As an example, mRNAs enriched in binding to RPL10A/uL1-containing ribosomes are shown to require RPL10A/uL1 for their efficient translation. Within several of these transcripts, this level of regulation is mediated, at least in part, by internal ribosome entry sites. Together, these results reveal a critical functional link between ribosome heterogeneity and the post-transcriptional circuitry of gene expression.

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Alternate JournalMol. Cell
PubMed ID28625553
PubMed Central IDPMC5548184
Grant ListDP2 OD008509 / OD / NIH HHS / United States
R01 HD086634 / HD / NICHD NIH HHS / United States
P50 GM107615 / GM / NIGMS NIH HHS / United States
R01 DK101743 / DK / NIDDK NIH HHS / United States
R01 DK106241 / DK / NIDDK NIH HHS / United States
R21 HD086730 / HD / NICHD NIH HHS / United States
P30 CA124435 / CA / NCI NIH HHS / United States

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