The Blood Transcriptome of Experimental Melioidosis Reflects Disease Severity and Shows Considerable Similarity with the Human Disease.

TitleThe Blood Transcriptome of Experimental Melioidosis Reflects Disease Severity and Shows Considerable Similarity with the Human Disease.
Publication TypeJournal Article
Year of Publication2015
AuthorsConejero L, Potempa K, Graham CM, Spink N, Blankley S, Salguero FJ, Pankla-Sranujit R, Khaenam P, Banchereau JF, Pascual V, Chaussabel D, Lertmemongkolchai G, O'Garra A, Bancroft GJ
JournalJ Immunol
Date Published2015 Oct 01
KeywordsAnimals, Arginase, Burkholderia pseudomallei, Disease Models, Animal, Female, Gene Expression Profiling, Humans, Immunity, Innate, Interferon-gamma, Interleukin-10, Lung, Matrix Metalloproteinase 9, Melioidosis, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Nitric Oxide Synthase Type II, Receptors, Immunologic, Signal Transduction, Transcriptome, Triggering Receptor Expressed on Myeloid Cells-1

Melioidosis, a severe human disease caused by the bacterium Burkholderia pseudomallei, has a wide spectrum of clinical manifestations ranging from acute septicemia to chronic localized illness or latent infection. Murine models have been widely used to study the pathogenesis of infection and to evaluate novel therapies or vaccines, but how faithfully they recapitulate the biology of human melioidosis at a molecular level is not known. In this study, mice were intranasally infected with either high or low doses of B. pseudomallei to generate either acute, chronic, or latent infection and host blood and tissue transcriptional profiles were generated. Acute infection was accompanied by a homogeneous signature associated with induction of multiple innate immune response pathways, such as IL-10, TREM1, and IFN signaling, largely found in both blood and tissue. The transcriptional profile in blood reflected the heterogeneity of chronic infection and quantitatively reflected the severity of disease. Genes associated with fibrosis and tissue remodeling, including matrix metalloproteases and collagen, were upregulated in chronically infected mice with severe disease. Transcriptional signatures of both acute and chronic melioidosis revealed upregulation of iNOS in tissue, consistent with the expression of IFN-γ, but also Arginase-1, a functional antagonist of the iNOS pathway, and was confirmed by immunohistochemistry. Comparison of these mouse blood datasets by pathway and modular analysis with the blood transcriptional signature of patients with melioidosis showed that many genes were similarly perturbed, including Arginase-1, IL-10, TREM1, and IFN signaling, revealing the common immune response occurring in both mice and humans.

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Alternate JournalJ. Immunol.
PubMed ID26311902
PubMed Central IDPMC4576736
Grant List294682 / / European Research Council / International
P30 CA034196 / CA / NCI NIH HHS / United States
U117565642 / / Medical Research Council / United Kingdom
MC_U117565642 / / Medical Research Council / United Kingdom
085162 / / Wellcome Trust / United Kingdom

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